hbv dna quantitative normal range

These HBeAg-negative and anti-HBe–positive patients have serum HBV DNA levels usually lower than those in patients who are HBeAg-positive with chronic hepatitis B. European Association For The Study Of The Liver EASL clinical practice guidelines: Management of chronic hepatitis B virus infection. -. Hepatitis B surface antigen quantification: why and how to use it in 2011—a core group report. The progression to a state of detectable liver injury is characterized by the presence of HBsAg and hepatitis B e antigen (HBeAg) in serum, moderate to high levels of circulating HBV DNA, elevation of serum ALT levels, and absence of antibody against HBeAg (anti-HBe). Found inside – Page 171Normal Name Reference Range Indication Interpretation HBsAg Negative Pregnancy HBsAg appears between 6 and 16 weeks ... Patients with a positive HBsAg should have a quantitative HBV DNA, HBeAg, and ALT at baseline and again at 28 weeks. Do you want to know the details that should be taken into consideration in order to have accurate conventional and real-time PCR results? If so, this book is for you. It is used to assess recovery from infection and candidacy for antiviral therapy and to differentiate between inactive carrier state and chronic active hepatitis in chronic HBV infection. 119 Ali Koyuncuer: Associations between HBeAg Status, HBV DNA, ALT Level and Liver Histopathology in Patients with Chronic Hepatitis B and AFP were 32.6 ±21.0 IU/L (range 8 to 113 IU/L), 25.6 The Cobas Taqman assay for hepatitis B virus (HBV) DNA showed linear detection over 7 logs for genotypes A to D. The coefficient of variation was 1.2% at ≥1,000 IU/ml and 22.0% at 10 IU/ml. What is the normal reference range for an HBV DNA Quantitative PCR Test? Prior to integrating the HBsAg quantitative assay into our clinical practice, we relied exclusively on HBV DNA decline to predict response in chronic hepatitis B patients treated with peginterferon alfa-2a. Elevated serum HBV DNA levels at study entry were associated with an increased risk of HCC after multivariate adjustment for risk predictors including HBV genotype and mutants (odds ratio 1.6 for HBV DNA levels ≥105 versus 104 to <105 copies/mL, P = 0.04). The chronic hepatitis B phase was determined for each patient after evaluating liver function test over one year, HBe antigen status and HBV DNA levels as: Immune tolerant (IT) phase, defined as HBeAg positive, high viral load ( >20000IU/ml), serum ALT <2X upper limit normal (ULN), Immune clearance (IC) phase, defined as HBeAg positive, HBV DNA >20000IU/ml, serum ALT >2 X ULN, Low replicative . Results are reported in International Units per milliliter (IU/mL). Once it was agreed that the laboratory would invest in the necessary equipment, we were able to work with the virologists and the viral hepatitis team to integrate the test as part of our standard laboratory investigations. However, many of these studies were limited by small number of cases and controls, inadequate matching or adjustment of confounding factors, lack of causal temporality, and analysis of risk predictors at study entry only. i am hbv carrier please tell to normal range FINAL TEST REPORT Spec.type Test Name Results Units Normal Ranges VIROLOGY SERUM HBeAg Nonreactive(0.66) S/CO index 1.0 REACTIVE Cobas e411 ECLIA/Architect CMIA METHOD MOLECULAR BIOLOGY PLASMA HBV DNA Quantitative test * 2,129 copies/ml RealTime HBV assay is intended for use in conjunction with clinical presentation and laboratory markers as an . • In anti-HBe-positive patients, HBV DNA levels positively correlated with HAI-NI (r=0.31; • Serum HBV DNA levels significantly correlated with necroinflammatory score (r=0.59; 213 patients with chronic hepatitis B and serum HBV DNA level >10, • Serum HBV DNA levels were not correlated with histological grade and stage of liver disease in both HBeAg-positive and -negative patients, 40 HCC patients underwent surgical resection, 125 HCC patients underwent systemic chemotherapy, Real-time PCR (LOQ: 10 copies/mL) for pretreatment HBV DNA levels, 62 HCC patients underwent transarterial chemolipiodoliza-tion, HBeAg, Child-Pugh classification, number of tumors, and Lamivudine use, Age, AFP, Child-Pugh classification, tumor size, number of tumors, and Lamivudine therapy, 3653 HBsAg-positive, Anti-HCV-negative adults from community, Sex, age, cigarette smoking, alcohol consumption, HBeAg, ALT level, and cirrhosis status, 157 patients with HCC underwent surgical resection (excluded patients with fluctuating DNA), Tumor size, tumor number, vascular invasion, and grades, 72 HCC patients underwent surgical resection, Age, AFP, tumor size, tumor differentiation, lymphovascular permeation, and microsatellite lesions, 69 patients with HCC metastasis or recurrence in two years of follow-up, GGT, tumor capsule, portal vein thrombosis, tumor dimension, cirrhosis, BCLC stages, and BCP mutations, 70 patients with HBeAg-positive chronic hepatitis, 11 patients with liver-related death (HCC and liver failure) and 1 with orthotopic liver transplantation, Sustained remission HBeAg-/HBV DNA+ or HBeAg reversion and HBeAg persistence, Age, sex, duration of HBeAg positive phase, and cirrhosis at entry, 436 patients with advanced fibrosis or cirrhosis receiving Lamivudine and 215 patients receiving placebo, Overall disease progressionIncrease in Child-Pugh scoreHCC, LamivudinePlaceboLamivudinePlacebo LamivudinePlacebo, 1.0 (referent)0.5 (0.3–0.7)1.0 (referent)0.5 (0.2–0.9) 1.0 (referent)0.5 (0.3–1.0), Country, sex, baseline ALT level, Child-Pugh score, and Ishak fibrosis score, 353 patients with chronic hepatitis and 303 patients with cirrhosis receiving Lamivudine treatment, Maintained virological responseVirological breakthrough, Sex, age, initial diagnosis, baseline HBV DNA levels, baseline ALT levels, hepatic flare after virological breakthrough, and previous IFN therapy. In multivariate Cox regression analyses of risk factors predicting progression to liver cirrhosis, increasing HBV DNA level was the strongest independent predictor.13, The findings from case-control and case-cohort studies on the association between serum HBV DNA levels and risk for HCC are summarized in Table 2. Hepatitis B is a STD that is caused by the Hepatitis B virus (HBV). The HBV quantitative real-time PCR assay has a quantitative range of 10 to 1,000,000,000 IU/mL. It may also be used to detect if there was an infection in the past. About 5-10% of. The HBsAg quantitative assay records the amount of hepatitis B surface antigen in a patient's blood. Keywords: Found inside – Page 1269Clinical and Histologic Diagnosis at Presentation Anti - HBe / HBV - DNA positive Anti - HBe / HBV - DNA negative 81 ... Results Anti - HBe / HBV - DNA Carriers Assays Standard laboratory procedures were used to test liver function . We performed HBV DNA quantitative detection of HBV samples and compared the results of our duplex real-time PCR assays with the COBAS TaqMan HBV Test version 2 and Daan real-time PCR assays. Please check your email for instructions on resetting your password. 2500 Rs. The observed benefits of treatment was most likely driven by viral suppression, because disease progression among treated patients who developed lamivudine resistance (YMDD mutants) was 13%, which was higher than that in the group without drug resistance. 976 HBV related patients were analysed in this retrospective cross-sectional study. Symptoms of Hepatitis B are: appetite loss, low-grade fever, muscle and joint aches, nausea and vomiting, yellow skin and dark urine due to jaundice. The quantification range of this assay is 10 to 1,000,000,000 IU/mL (1.00 log to 9.00 log IU/mL). Read More »Hbv Quantitative Real Time Pcr Normal Range. An "Undetected" result indicates that hepatitis B virus (HBV) DNA was not detected in the serum specimen. J Hepatol. The method used for HBV DNA Viral Load PCR is COBAS TaqMan HBV Test. cobas® 4800/6800/8800 Systems. Successful treatment is associated with a reduction in liver injury and fibrosis (scarring), a decreased likelihood of . During this period, serum HBV DNA levels are persistently high. IU/µl. Background/aims: To investigate whether the measurement of HBV DNA by quantitative polymerase chain reaction (PCR) is helpful in monitoring response to interferon treatment in chronic hepatitis B virus infection, we have determined sequentially serum levels of HBV DNA during and up to 18 months after treatment, in 10 patients with a sustained response (all anti-HBe positive, five also HBsAg . The HBV DNA levels of six samples were undervalued by duplex real-time PCR assays of the C region because of mutations in the primer of C region. normal ALT (Table 1). Correlation with HBV DNA quantitative levels may help in developing strategies for antiviral treatment. During each cycle the fluorescence is measured, which greatly increases the dynamic range of the . 29,000) cp/mL] than in chronic hepatitis B cases [2,800,000 (range, 1400-1,200,000,000) cp/mL] (P 0.001). The quantification range of this assay is 20 to 170,000,000 IU/mL (1.30-8.23 log IU/mL). If you have a viral load of 105 copies/mL, it is actually, 10 X 10 X 10 X 10 X . 2014 Jun;201:24-30. doi: 10.1016/j.jviromet.2014.01.015. - Find out the costs to your organisation of carrying out the test. Bookshelf 2012;142:1303–13. Consequently, treatment discontinuation was often restricted to those patients experiencing adverse effects while taking the drug. Article Google Scholar 53. Similarly, HBV DNA levels at the start of observation are thought to be associated with hepatocarcinogenesis. The other authors declare that they have no potential conflicts of interest. 2021 Aug;58:101748. doi: 10.1016/j.mcp.2021.101748. The quantification range of this assay is 10 to 1,000,000,000 IU/mL (1.00 log to 9.00 log IU/mL). Another group of infected persons is able to inactivate the infection and go into the “nonreplicative phase” or “inactive carrier state.” Patients in this phase are characterized by the continued presence of HBsAg in serum, absence of HBeAg and presence of anti-HBe, low levels of serum HBV DNA and normal serum ALT values. The quantification range of this assay is 20 to 170,000,000 IU/mL (1.30-8.23 log IU/mL). Found inside – Page 6057J Virol 1994 Nov ; 68 ( 11 ) : 7591-7 El protein of human papillomavirus type la is sufficient for Stabilities of quantitative plasma culture for human Quantitative detection of hepatitis B virus DNA in serum initiation of viral DNA ... Probes are used to detect and quantify, but Hepatitis B Virus DNA (HBV DNA) Testing and Interpretation Update Page 1 of 3 LAB-SD-031-003 . Monitoring of viral load may be helpful in making or altering treatment regimens in HBV infected patients. Print Share Include LOINC® in print. Just another summarized diary . In a hospital-based nested case-control study on 48 patients with HCC and 48 age-matched and sex-matched controls with cirrhosis in Japan, patients with HCC had higher serum HBV DNA levels at study entry than patients with cirrhosis who remained HCC-free during follow-up.18 In three case-control studies in which the serum HBV DNA levels at study entry were dichotomized into two groups, the elevated HBV DNA level group in each study had an increased risk of developing HCC: the multivariate-adjusted odds ratio comparing high versus low HBV DNA levels being 15.6 (≥83 versus <83 copies/mL) in a study from Senegal;19 3.1 (≥83 versus <83 copies/mL) in a study from China,19 and 2.5 (≥105 versus <105 copies/mL) in a study from Taiwan.21, Nested in a large-scale hospital-based long-term follow-up study of 4841 male HBV carriers in Taiwan, a case-control study of 154 newly developed HCC cases and 316 matched HBV carrier controls found a significantly increasing risk of HCC across the biological gradient of serum HBV DNA levels at study entry.20 In the further analysis of HBV DNA levels in serial serum samples collected during follow-up, a significant dose-response relationship was observed between the risk of HCC and the proportion of follow-up serum samples with elevated HBV DNA levels.24 The significant dose-response relationship was also observed in another community-based nested case-control study of 170 newly diagnosed cases of HCC and 276 HBV carrier controls with normal ALT levels at study entry.23 In a hospital-based cross-sectional case-control study of 183 cases of HCC and 202 HBV carrier controls, serum HBV DNA levels were sigificantly associated with HCC risk in the older (> 40 years) but not in the younger (≤ 40 years) age group.22, The findings of cohort studies on the association between serum HBV DNA levels and HCC risk are summarized in Table 3. The HBV DNA test is performed on a blood sample using a Polymerase Chain Reaction (PCR) technique that rapidly generates HBV DNA fragments so they can be measured. An interpretation of "Not Detected" does not rule out the presence of inhibitors in the patient specimen or HBV DNA concentration below the level of detection of the test. Home; About; Blog; Search for. Methods. A single . Serum HBV-DNA Levels in Inactive Hepatitis B Virus Carriers Dear Sir: . This test helps determine whether Hepatitis B Virus is getting reproduced in the liver. Quantitative measurement of HBV viral DNA may be used to monitor progression of disease. 2011 May 14;8:227. doi: 10.1186/1743-422X-8-227. Hepatitis B Virus DNA, Ultra Sensitive Quantitative PCR Label Mnemonic: HBVQUANT : Epic code: LAB3284: Order form: Microbiology/Molecular Infectious Disease Requisition: Specimen(s): Plasma. In 97 clinical samples, the log HBV DNA/ml differed by 0.11 between Cobas Amplicor and Cobas Taqman (r2 = 0.97). A quantitative result expressed in IU/mL (International Units Per Millilitre) indicates the degree of active HBV viral in the patient. HBV DNA . The evidence for an association between serum HBV DNA levels and histopathological findings on liver biopsies are summarized in Table 4. Quantification Hepatitis B virus (HBV) DNA plays a critical role in the management of chronic HBV infections. Mani K, Thirumalmuthu K, Kathiresan DS, Ramalingam S, Sankaran R, Jeyaraj S. Mol Cell Probes. Wang CC, Lim LY, Deubner H, et al. The following institutions and investigators, in addition to the authors, participated in the REVEAL-HBV Study Group as a part of Taiwan Community-Based Cancer Screening Project: Chang-Gung Memorial Hospital and Chang-Gung University (Y. F. Liaw); Genomics Research Center (C. L. Jen, S. L. You); College of Public Health, National Taiwan University (T. H. H. Chen); Department of Gastroenterology, Kaohsiung Chang-Gung Memorial Hospital (S. N. Lu); Department of Microbiology, National Taiwan University (S. H. Yeh); Department of Public Health, National Defense Medical Center (C. A. These findings raise the question if there is enough evidence that persistent elevations in HBV DNA levels over time (rather than a single elevated value) is a stronger risk predictor for disease progression. If HBV viral load is greater than 20,000 international units per milliliter in a person with detectable Hepatitis B envelope antigen then it indicates that the virus is active and has . Baseline serum HBV- DNA levels less than 100,000 cp/mL were detected in 17 (12.7%) and less than 30,000 cp/mL in 14 (10.5%) of 134 chronic hepatitis B patients, while all inactive carriers had baseline viremia . Even after the relatively brief treatment period of 36 months, lamivudine treatment was associated with fewer cases of HCC than was placebo treatment (7.4% versus 3.9%), although this difference was just short of statistical significance. Dynamic comparison between Daan real-time PCR and Cobas TaqMan for quantification of HBV DNA levels in patients with CHB. The importance of serum HBV DNA levels as a predictor of the development of cirrhosis and HCC has been extensively reviewed recently.6 Several hospital-based and community-based case-control and cohort studies have consistently found significant associations between elevated HBV DNA levels and risk of liver cirrhosis and HCC. Learn about our remote access options, Genomics Research Center, Academia Sinica, National Taiwan University, Taipei, Taiwan, Graduate Institute of Epidemiology, College of Public Health, National Taiwan University, Taipei, Taiwan, Global Epidemiology and Outcomes Research, Pharmaceutical Research Institute, Bristol-Myers Squibb Co., Wallingford, CT, Other members of the REVEAL-HBV (Risk Evaluation of Viral Load Elevation and Associated Liver Disease/Cancer HBV) Study Group are listed in the Acknowledgment. A quantitative result expressed in IU/mL (International Units Per Millilitre) indicates the degree of active HBV viral in the patient. The aim of this study is to reveal the clinical and histopathological features of HBsAg-positive and HBeAg-positive chronic hepatitis B infected patients with high level of HBV DNA, from 17 hospitals and medical centres in China, with alanine aminotransferase levels within the lower region of normal range versus those with levels within the upper region of normal range and to investigate the . The linear range (analytical measurement) of the artus HBV RG PCR Kit was determined by analyzing a dilution series of a HBV quantitation standard ranging from 1 x 10. In another three case-control studies on death from HCC, patients with high serum HBV DNA levels at study entry had a significantly higher risk of HCC death than those with low serum HBV DNA levels.35, 36, 41 A dose-response relationship between serum HBV DNA levels and the metastasis or recurrence of HCC was reported in another case-series study.40. The Royal London Hospital, Barts Health NHS Trust. If a mutation caused a sequence mismatched in the primer or probe of a commercial DNA quantification kit, this would lead to an underestimation . This is consistent with new AASLD guidelines and recent data from Lin et al , which correlate parameters of progressive disease with high normal ALT. Role of the Sponsors: Dr. Iloeje (employed by Bristol-Myers Squibb) was involved in the study design, development of the analysis plan, and interpretation of the data and writing of the report. Collection Medium: Pink top tube 6 mL (K2-EDTA) Minimum: One full pink top tube EDTA anti-coagulated pink top tube. Found inside – Page 2351During early chronic HBV infection, HBV DNA can be detected both in serum and in hepatocyte nuclei, ... This substitution results in the replacement of the TGG tryptophan codon by a stop codon (TAG), which prevents the translation of ... (HEPATOLOGY 2009;49:S72–S84.). (022)48937160. Interventional studies have shown a strong correlation between the indices of disease activity seen on liver biopsy and levels of serum HBV DNA. The evidence for an association between serum HBV DNA levels and histopathological findings on liver biopsies are summarized in Table 4. 2014 Nov 1;437:168-74. doi: 10.1016/j.cca.2014.07.021. The . Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC . In-silico analysis of Covid-19 genome sequences of Indian origin: Impact of mutations in identification of SARS-Co-V2. Quantitative HBsAg testing demonstrates both when peginterferon alfa-2a is providing some treatment benefit and when a virological response is unlikely. 8. 2021 Jan 19;21(1):83. doi: 10.1186/s12879-020-05747-4. HBV testing cannot be added onto a previously opened . In another intervention study of lamivudine therapy in 353 patients with chronic hepatitis B and 303 patients with cirrhosis, the risk of developing HCC or liver-related death was three-fold higher among patients with cirrhosis who had virological breakthrough than in those who achieved persistent viral suppression on treatment.43. HBV Quantitation; HBV Viral Load; Expected Turnaround Time . Found inside – Page 260The results showed that SAT-EV71 has a high sensitivity and specificity: it detected the VP1 with a minimum of 10 copies per ... Researchers reported a novel approach for both qualitative and quantitative analysis of HBV DNA (76). If a mutation caused a sequence mismatched in the primer or probe of a commercial DNA quantification kit, this would lead to an . There are an estimated 350 million carriers of hepatitis B surface antigen (HBsAg) in the world among whom there are more than 500,000 deaths annually attributable to cirrhosis and liver cancer (World Health Organization and Centers for Disease Control and Prevention fact sheets are available at www.who.int and www.cdc.gov). The biological gradient remained significant in stratified analyses across a variety of baseline characteristics such as sex, age, and habits of cigarette smoking and alcohol drinking. The Quantitation of HBV DNA (IU/mL) is achieved by amplifying in each run standard curves consisting of serial dilutions of a known number of DNA containing the HBV amplicons. Hepatitis B quantitative DNA PCR can be used in conjunction with clinical presentation and other laboratory markers of disease status as an aid in managing individuals infected with HBV. Nevertheless, the potentially adjuvent role of pregnancy and HBV-DNA was still dete ctable by quantitative PCR (4 14-226,138 copies/ AZT must be discussed, ml) in 17/38 (45%) patients. An "Undetected" result indicates that hepatitis B virus (HBV) DNA was not detected in the serum specimen. A significant association between elevated serum HBV DNA levels and increased risk of HCC was observed in all studies despite differences in study design (cross-sectional versus longitudinal and community/hospital–based), health status of controls, method and detection limit for the determination of HBV DNA levels, HBV DNA levels selected as the referent group, and variables chosen for adjustment in the analyses.17-24 In a community-based, nested case-control study of 44 HBeAg-negative patients with newly developed HCC and 86 matched controls who were selected from a cohort of 1991 male HBeAg-negative HBV carriers in Taiwan, a significant dose-response relationship between serum HBV DNA levels at study entry and HCC risk was observed.17 Compared with serum HBV DNA levels <2.5 pg/mL as the referent group, the multivariate-adjusted odds ratio was 2.3 and 6.0, respectively, for serum HBV DNA levels of 2.5-13.0 and >13.0 pg/mL. Hepatitis B. If you have a viral load of 105 copies/mL, it is actually, 10 X 10 X 10 X 10 X . Instead of writing 100,000 copies/mL, labs may report it as one to the fifth power or 105 or 5 log. TEST: 551722 . It is a nucleotide chemical modification technology (also known as 3rd generation reverse oligonucleotide) . 2012;57:167–85. Methods. The results of 19 cases with normal serum from the two methods were below the minimum cut-off value (TaqMan: 80 IU/ml and LNA: 40 IU/ml). The correlation and bland-altman analysis of the quantitative results of S and C regions of duplex real-time PCR assays in 238 HBV samples. An "Undetected" result indicates that hepatitis B virus (HBV) DNA was not detected in the serum specimen. The distributions of serum HBV DNA levels at study entry for participants who were HBeAg-seronegative and with normal ALT levels (<45 U/L) without cirrhosis are shown in Fig. A result of "<10 IU/mL (<1.00 log IU/mL)" indicates that HBV DNA is detected, but the HBV DNA level present cannot be quantified accurately below this lower limit of quantification of this . Patients with chronic liver disease of unknown origin most commonly have HBV that is detected by viral DNA testing. The corresponding author had final responsibility for the decision to submit for publication. We can help you find the right test or let you . 2020 Jun 10;7(6):200636. doi: 10.1098/rsos.200636. Background: ALT levels should remain consistently within the normal range, and HBV DNA should be below 2000 IU/ml 7. HBV genotype C infection was associated with a higher risk of HCC than HBV genotype B. Clin Liver Dis. In the REVEAL-HBV study,14 the mortality (per 100,000 person-years) increased with baseline HBV DNA level (in copies/mL) ranging from 9 (<300), 48 (300-9.9 × 103), 75 (1.0 × 104-9.9 × 104), 143 (1.0 × 105-9.9 × 105), to 267 (≥ 1 × 106) for chronic liver disease and cirrhosis; and 73, 48, 174, 692, and 816, respectively, for liver cancer. In a case-control study of 79 patients with cirrhosis and 158 controls matched on age, sex, and HBeAg serostatus, the presence of cirrhosis was associated with higher levels of serum HBV DNA.15 In a follow-up study of 2763 HBsAg-seropositive adults in China, elevated serum HBV DNA levels at study entry were associated with an increased mortality from chronic liver diseases and an increased morbidity of severe liver diseases among survivors.16 In the REVEAL-HBV study, the incidence of cirrhosis (per 100,000 person-years) increased with increasing serum HBV DNA levels (copies/mL) at study entry: ranging from 339 (<300), 430 (300-9.999 × 103), 774 (1.0 × 104-9.9999 × 104), 1879 (1.0 × 105-9.99999 × 105) to 2498 (≥1 × 106). This, in combination with hepatitis B virus DNA (HBV DNA) testing, enables physicians to monitor and evaluate a patient's response to peginterferon alfa-2a therapy, and to stop treatment after 24 weeks if indicated. The evidence for an association between serum HBV DNA levels and histopathological findings on liver biopsies are summarized in Table 4.29-33 In a cross-sectional study of 55 patients who were HBeAg-negative with chronic hepatitis B, patients with detectable HBV DNA levels (≥ 0.5 mEq/mL) had significantly higher necroinflammation scores in histology activity index (HAI) than those with undetectable HBV DNA levels.29 In a cross-sectional study of 94 chronic hepatitis B patients,31 serum HBV DNA levels were positively correlated with necroinflammation scores of HAI (r = 0.31, P = 0.014), fibrosis scores (r = 0.33, P = 0.017), and total HAI scores (r = 0.37, P = 0.008) in patients who were positive for anti-HBe but not for those who were HBeAg-positive. 2016 Feb;111(2):134-40. doi: 10.1590/0074-02760150415. Geneva: World Health Organization; 2015. This, in combination with hepatitis B virus DNA (HBV DNA) testing, enables physicians to monitor and evaluate a patient's response to peginterferon alfa-2a therapy, and to stop treatment after 24 weeks if indicated. HBV (Hepatitis B Virus) Viral Load Quantitative Test Lab Address in Nagpur. In mathematical jargon, a "log" equals a number multiplied by 10. 2013;2:8-10. Epub 2014 Jul 24. The relative importance of serial HBV DNA measurements, the loss of hepatitis B e and surface antigens, as well as the emergence of HBV mutants in the progression of chronic hepatitis B, especially in young patients, is an important need for future research. A Hepatitis B Virus test also known as HBV test is a blood test that determines whether there is an active hepatitis B infection. The quantification range of this assay is 10 to 1,000,000,000 IU/mL (1.00 log to 9.00 log IU/mL). Found inside – Page 219Table 3.4 Interpretation of results of serological tests for hepatitis B HBsAg HBeAg Anti-HBe Anti-IgM HBcIgG ... Quantitative tests for HBV DNA are limited by a lack of standardisation of the assays and of widely accepted HBV DNA ... The dilution series was calibrated against the WHO 1st International HBV DNA Standard. Found inside – Page 173... Number of HBV DNA molecules before amplification FIGURE 5 The dynamic range and sensitivity of the quantitative test ... The quantitative results from the two tests were in good agreement for all samples , demonstrating that the PCR ... Subscribe. In a cross-sectional study of 47 HBsAg-positive blood donors with detectable serum HBV DNA levels, serum HBV DNA levels significantly correlated with HAI-necroinflammation scores (r = 0.59; P < 0.001) and Ishak fibrosis stages (r = 0.50; P < 0.001).32 In another cross-sectional study of 213 patients with chronic hepatitis B with serum HBV DNA level >105 copies/mL, serum HBV DNA levels were not correlated with histological grade and stage of liver disease in either HBeAg-positive and HBeAg-negative patients.33 In a large meta-analysis of 26 intervention studies including 3428 treated patients, histological grades were significantly associated with serum HBV viral level at study entry (r = 0.78; P = 0.0001) and at the end of treatment (r = 0.71; P = 0.003).30 More importantly, improvement in histological grade was strongly correlated with a decrease in serum HBV DNA levels (r = 0.96; P < 0.001). Sun S, Meng S, Zhang R, Zhang K, Wang L, Li J. Virol J. Thereafter, the inactive HBV carrier with undetectable or very low HBV DNA levels should be followed up with ALT determinations every 6 months after the first year and periodical measurement of HBV DNA levels 6 for the rest of their lifetime. Patients and Methods: A total of 106 treatment- naïve, HBeAg −ve HBV/D patients were included; 78 in the inactive carrier (IC) state and 28 in the active hepatitis (AH) stage. Chronic hepatitis B virus (HBV) infection is a global public health concern. HEPATITIS B VIRAL DNA (HBV DNA) QUANTITATIVE, REAL TIME PCR. This test is intended for use as an aid in the management of patients with chronic HBV infection undergoing anti-viral therapy. Proposed cutoffs for consideration for antiviral therapy is 100,000 copies/mL or 20,000 IU/mL in HbeAg-positive patients with .

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